RNA Preparation and Purification
Bioworld RNase AWAY™ 1L
RNase Away™ removes RNase and DNA contamination from glassware and plastic quickly and economically. Simply apply RNase away to surface or soak as needed and rinse with water. Non-abrasive formulation does not contain strong acids. Effective for use with electrophoresis equipment, glass plates, and other laboratory equipment. Specification: Removes RNase and DNA - Ready to use - Non-abrasive. Molecular Weight : 18.02 g/mol, pH : 6.0 - 8.0 at 25 °C, Solubility : completely miscible, Appearance Form : Liquid, Appearance Color : Clear, Melting Point : 0.0 °C, Boiling Point : 100.0 °C, Density : 1.023 g/cm3. 1L
New England Biolabs, Inc. NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module – 100 reactions
This module is part of the non-directional Ultra II RNA workflow, for Illumina- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.
- Designed for use in the NEBNext Ultra II non-directional RNA workflow
Research Products International Corp Direct-zol RNA MicroPrep w/Zymo-Spin IC Columns, Capped, 200 Preps
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis.
Active Motif Low Cell ChIP Kit 16 rxns
Active Motif's Low Cell ChIP-Seq Kit provides a complete ChIP-Seq workflow for chromatin preparation, immunoprecipitation and NGS library preparation from limited amounts of cells or small tissue biopsies. The assay is robust enough to work with both high abundance histone modifications and low abundance transcription factor proteins. The Next Gen DNA Library and Next Gen DNA Indexing reagents are included so you can generate high complexity sequencing libraries that are compatible with Illumina platforms.
Zyagen Labs Mouse C57 Brain Total RNA, 0.05 mg
Mouse C57 Whole Brain Total RNA is extracted from freshly harvested normal healthy tissue using classical guanidine isothiocyanate–phenol: chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase-1 to remove residual DNA, precisely quantified by NanoDrop, and stored at -80oC.
New England Biolabs, Inc. NEBNext Cell Lysis Buffer Module – 96 reactions
This module contains the enzymes and buffers required to lyse isolated cultured and primary cells to extract RNA. The module is optimized for use with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina or NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module, and can be used to supplement the NEBNext Cell Lysis Buffer and Murine RNase Inhibitor components supplied in these products.
New England Biolabs, Inc. Monarch® Spin Columns S2A and Tubes - 100 preps
The Monarch Spin Columns S2A and Tubes are a component of Monarch kits for RNA cleanup, and also offered separately for convenience and flexibility. The columns are designed without the use of a retaining ring, helping to ensure no buffer retention and no carryover of contaminants.
- Monarch spin columns and collection tubes designed for nucleic acid purification
- Minimal to no buffer retention/risk of carry-over contamination
- Low elution volume from unique column design
- Included in Monarch kits, also offered separately for flexibility
- Made with less plastic than conventional columns from leading suppliers
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) – 6 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA.
STEMCELL Technologies Total RNA Purification Kit, Size: 50 Preparations
The Total RNA Purification Kit uses a membrane-based system to quickly purify intact total RNA from 1 x 10^2 to 5 x 10^6 cells without an organic extraction step, eliminating the need for additional purification steps. The Total RNA Purification Kit includes an on-column DNase I treatment to significantly reduce genomic DNA contamination and ensure the purified RNA is suitable for sensitive downstream applications. Intact total RNA can be isolated in as little as 30 minutes. The Total RNA Purification Kit is not recommended for the purification of small RNAs (< 200 nt), such as miRNA.
Biochain Institute Inc Total RNA - Mouse Normal Tissue: Pancreas, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Plant Normal Tissue: Soy bean, 100 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue 5 Donor Pool: Liver, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
New England Biolabs, Inc. NEBNext Multiplex Small RNA Library Prep Kit for Illumina Set 2 – 96 reactions
The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext Small RNA Library Prep Set for Illumina (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing.